Laboratory Testing of WaterBug Samples

Volatile Organic Compounds (VOCs)

Volatile Organic Compounds are tested using EPA Method 8260B. A one gram sample of the WaterBug material is cut from the larger main sample and placed into a pre-cleaned 40 ml vial used for analysis. Then, five ml of filtered water is added to the 40ml vial and then placed in the auto sampler. This is repeated for all samples, with tools used for the weighing process (tweezers/scissors) being rinsed with filtered water between samples. A Laboratory Control Sample is analyzed with each analytical batch and the results are reported. Method Blank and Laboratory Control Samples (LCS) are weighed in the same manner using 1 gram of matching WaterBug material. A Matrix Spike Sample (MS) and Matrix Spike Duplicate (MSD) are prepared and analyzed for each batch of sampling received. Five ml of filtered water along, with the surrogate and internal standard, are added to all samples by the instrument to complete quality control (QC). The samples are then analyzed using an Agilent 6890 GC equipped with a 5937 MSD for VOC analysis.

Semi-Volatile Organic Compounds (SVOCs) and Diesel Range Organics (DROs)

Semi-Volatile Organic Compounds are tested using EPA Method 8270C. Diesel Range Organics are tested using EPA Method 8015B.

The Open-Cell foam matrix is prepared for analysis by shaking in chromatography-grade hexanes. Five grams of the Open-Cell foam matrix is weighed into a pre-cleaned 2 oz. sample collection jar that is labeled with the sample ID. This is repeated for all samples, with tools used for the weighing process (tweezers/scissors) being rinsed with solvent between samples. Method Blank and Laboratory Control Samples are weighed in the same manner using 2 grams of matching Open-Cell foam matrix.

In the fume hood, a funnel containing a phase separator filter paper and a 60 mL receiver are thoroughly rinsed with methylene chloride. The receiver is labeled with sample IDs and the funnel is set up to drain directly into the receivers.  Each sample is lined up with the corresponding receiver and the lids are removed. Each sample and QC sample is spiked with surrogate.  The surrogate is slowly spiked directly onto the Open-Cell foam matrix so that it is adequately absorbed.  The LCS is spiked with matrix spike in the same manner. Ten mL of hexanes is then slowly added to each sample and the samples are capped and shaken by hand for two minutes. Samples are lined back up with their corresponding receivers.

The samples are individually decanted into the funnels. A clean disposable glass pipette is used to thoroughly press the hexanes from the Open-Cell foam matrix inside of the jar. Hexanes are added to the funnel assembly, and 10mL of fresh hexane is added to the sample in the jar. Prior to shaking the samples again, the pipette is used to press the Open-Cell foam matrix so that it absorbs fresh hexane. The samples are hand shaken for an additional 2 minutes, and are decanted in the same manner as the described originally. Enough hexanes are added to the sample to soak the Open-Cell foam matrix and rinse the jar. The Open-Cell foam matrix is thoroughly pressed so that it is as dry as possible. This final rinse is decanted into the funnel. The funnel assembly is rinsed with hexanes.

Samples are concentrated to 1 mL final volume using a rotary evaporator (N-EVAP). The final volume is achieved using a Class A 1 mL volumetric flask. The 1 mL samples are transferred into labeled GC vials for analysis by their respective methods (8270C for Semi-Volatiles and 8015B for Diesel Range Organics) using an Agilent 6890 GC equipped with a 5975 MSD for SVOC analysis, and a FID for DRO Analysis.